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KMID : 1007520140230010215
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Food Science and Biotechnology
2014 Volume.23 No. 1 p.215 ~ p.219
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Cytotoxicity of the White Ginseng Extract and Red Ginseng Extract Treated with Partially Purified β-Glucosidase from Aspergillus usamii KCTC 6954
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Jo Mi-Na
Jung Ji-Eun
Lee Jae-Hoon
Park Se-Ho
Yoon Hyun-Joo
Kim Kee-Tae
Paik Hyun-Dong
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Abstract
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Red ginseng extract (RGE) and white ginsengextract (WGE) were treated with partially purified β-glucosidase to increase a production of minor ginsenosides.The enzyme produced from Aspergillus usamii KCTC6954 was precipitated with (NH4)2SO4. Ginseng extractswere treated with a crude extract possessing β-glucosidaseactivity (1,089.2 μM/mL·min) at 60℃ for 72 h. The resultsof HPLC showed that enzyme-treated RGE and enzymetreatedWGE have increased amounts of minor ginsenosidescompared to each controls implying that the ginsenosideRb1 in WGE and RGE is converted enzymatically to Rd,F2, Rg3, and compound K. In cytotoxicity study, 2.5 mg/mLof RGE, 1.25 mg/mL of ERGE, and 5 mg/mL of WGE andEWGE were effective against the HepG2, AGS, and DLD-1, but HeLa and SK-MES-1 were not affected at anyconcentration. The results suggested that cytotoxicity ofginseng extracts treated with β-glucosidase were greaterthan that of each control against cancer cells.
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KEYWORD
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β-glucosidase, Aspergillus usamii, red ginseng, cytotoxicity, ginsenoside
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